Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with I-131 and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by I-131 and Tc-99m anti EGFr MoAb. Tc-99m labelling is very interesting. The tumor background ratio was 0.72 +/- 0.2 for Tc-99m and 0.40 +/- 0.6 for I-131 labelling. Moreover very high uptake of Tc-99m MoAb was obtained 2 hours after injection whereas the I-131 antibody required 24 hours.

In vivo study of a technetium labelled anti-EGFr MoAb

Danieli R;
1997

Abstract

Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with I-131 and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by I-131 and Tc-99m anti EGFr MoAb. Tc-99m labelling is very interesting. The tumor background ratio was 0.72 +/- 0.2 for Tc-99m and 0.40 +/- 0.6 for I-131 labelling. Moreover very high uptake of Tc-99m MoAb was obtained 2 hours after injection whereas the I-131 antibody required 24 hours.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12078/473
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact