Regular physical activity can enhance immune function and effectively prevents the spread of the cytokine response, thus reducing systemic low-grade inflammation and improving various immune markers. Moreover, regular exercise ameliorates antioxidant defense incrementally and diminish oxidative stress in skeletal muscle and other tissues, including immune cells. In this study we verified the efficacy of short-term endurance exercise in the adaptation of redox components in PBMCs and in the plasma cytokines. We performed a transcriptional and protein analyses of selected antioxidants (SOD1, SOD2, GPx1, TrxR1, CAT) and HSPs (HSP70, HSP27) in PBMCs from 10 physically active healthy males (26.63.1 years), also controlling for the level of 4- HNE and NFkB activation in PBMC, and for the protein carbonyls and cytokines’ concentration (IL6, IL8, IL10, IL17E, IL17F, IL21, IL22 and IL23) in plasma. Blood samples were collected before and after (3 and 24 hrs) an acute bout of endurance exercise (70% HRmax for 30’), or a short-term endurance training (70% HRmax for 30’/day for 5 consecutive days). A significant reduction in plasma protein carbonylation and PBMC 4-HNE was detected after 5-day training compared with the baseline levels (p<0.05). An early response (3hrs) in gene expression was detected for SOD1, Hsp70 and Hsp27 (p<0.05), followed by increase in protein levels at 24 hrs and return to basal level after 5 days, while a significant reduction in gene expression and/or protein content was identified for SOD2, GPx1 and TrxR1. This picture was paralleled by an increase in p-p65-NFκB at 24 hrs (p<0.05), its decrease below the baseline level at 5-day, concurrent with a decrease in the plasma level of the pro-inflammatory cytokines IL-8, IL-21 and IL-22. This study showed that 5 days of regular exercise is effective in improving the redox homeostasis in circulating PBMCs, as well as the systemic pro-inflammatory environment in healthy adults
Endurance exercise and immune function: role of redox homeostasis and inflammatory biomarkers in systemic adaptation
Guidotti, Flavia;
2021-01-01
Abstract
Regular physical activity can enhance immune function and effectively prevents the spread of the cytokine response, thus reducing systemic low-grade inflammation and improving various immune markers. Moreover, regular exercise ameliorates antioxidant defense incrementally and diminish oxidative stress in skeletal muscle and other tissues, including immune cells. In this study we verified the efficacy of short-term endurance exercise in the adaptation of redox components in PBMCs and in the plasma cytokines. We performed a transcriptional and protein analyses of selected antioxidants (SOD1, SOD2, GPx1, TrxR1, CAT) and HSPs (HSP70, HSP27) in PBMCs from 10 physically active healthy males (26.63.1 years), also controlling for the level of 4- HNE and NFkB activation in PBMC, and for the protein carbonyls and cytokines’ concentration (IL6, IL8, IL10, IL17E, IL17F, IL21, IL22 and IL23) in plasma. Blood samples were collected before and after (3 and 24 hrs) an acute bout of endurance exercise (70% HRmax for 30’), or a short-term endurance training (70% HRmax for 30’/day for 5 consecutive days). A significant reduction in plasma protein carbonylation and PBMC 4-HNE was detected after 5-day training compared with the baseline levels (p<0.05). An early response (3hrs) in gene expression was detected for SOD1, Hsp70 and Hsp27 (p<0.05), followed by increase in protein levels at 24 hrs and return to basal level after 5 days, while a significant reduction in gene expression and/or protein content was identified for SOD2, GPx1 and TrxR1. This picture was paralleled by an increase in p-p65-NFκB at 24 hrs (p<0.05), its decrease below the baseline level at 5-day, concurrent with a decrease in the plasma level of the pro-inflammatory cytokines IL-8, IL-21 and IL-22. This study showed that 5 days of regular exercise is effective in improving the redox homeostasis in circulating PBMCs, as well as the systemic pro-inflammatory environment in healthy adultsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
