Recent reports highlight the relevance of extracellularvesicles (EVs) as intercellular communication mediators ofsystemic adaptation induced by physical activity (PA). EVsare lipid-bound vesicles secreted by cells into the circulationand mediate intercellular communication by shuttlingfunctional molecules, such as different RNA species, lipids,DNA, and proteins. Taking into account the role of redoxhomeostasis in exercise-induced signaling and adaptation,we are interested in verifying the exercise-relatedintercellular communication of redox components mediatedby EVs’ cargo. Plasma EVs have been isolated from trainedand untrained healthy males (n= 14, 20-35 years) before andafter (3 and 24 hours) an acute bout of endurance exerciseor a short-term endurance training (5 days at 70% HRmax),and analyzed for their content in antioxidants and stressresponse proteins, as well as for specific myomiRs. Ourresults showed that plasma EVs contain a plethora ofproteins related to the oxidative stress response, such asCatalase (CAT), Glutathione Peroxidase 1 (GPX1),Thioredoxin reductase 1 (TrxR1), Thioredoxin 2 (Trx2)MnSOD (SOD2), Heat shock factor 1 (HSF1), p38-MAPK, aswell as miR-206, miR-133 and miR-1. Among all biomarkers,our data highlighted that SOD2 (p = 0.005), CAT (p = 0.005)and HSF1 (p = 0.023) content in EVs’ cargo is decreased intrained respect to the untrained subjects, with no effectsexerted by acute exercise. When untrained subjectsunderwent to 5-day endurance training, CAT (p = 0.03),SOD2 (p = 0.03) and HSF1 content were decreased,reaching levels similar to those found in trained. Finally, miR-206 increased in trained EVs 24h after the exercise (p =0.045). These results suggest that the protein and myomiRcontent in the EVs’ cargo could reflect the tolerance toexercise at systemic level determined by the well-knownadaptation of the redox homeostasis components ormyomiRs in response to exercise in skeletal muscle and other tissues.
Exercise-induced Modulation of Extracellular Vesicles' Cargo: a Focus on Antioxidants, Stress Proteins and miRNAs
Guidotti, F;
2020-01-01
Abstract
Recent reports highlight the relevance of extracellularvesicles (EVs) as intercellular communication mediators ofsystemic adaptation induced by physical activity (PA). EVsare lipid-bound vesicles secreted by cells into the circulationand mediate intercellular communication by shuttlingfunctional molecules, such as different RNA species, lipids,DNA, and proteins. Taking into account the role of redoxhomeostasis in exercise-induced signaling and adaptation,we are interested in verifying the exercise-relatedintercellular communication of redox components mediatedby EVs’ cargo. Plasma EVs have been isolated from trainedand untrained healthy males (n= 14, 20-35 years) before andafter (3 and 24 hours) an acute bout of endurance exerciseor a short-term endurance training (5 days at 70% HRmax),and analyzed for their content in antioxidants and stressresponse proteins, as well as for specific myomiRs. Ourresults showed that plasma EVs contain a plethora ofproteins related to the oxidative stress response, such asCatalase (CAT), Glutathione Peroxidase 1 (GPX1),Thioredoxin reductase 1 (TrxR1), Thioredoxin 2 (Trx2)MnSOD (SOD2), Heat shock factor 1 (HSF1), p38-MAPK, aswell as miR-206, miR-133 and miR-1. Among all biomarkers,our data highlighted that SOD2 (p = 0.005), CAT (p = 0.005)and HSF1 (p = 0.023) content in EVs’ cargo is decreased intrained respect to the untrained subjects, with no effectsexerted by acute exercise. When untrained subjectsunderwent to 5-day endurance training, CAT (p = 0.03),SOD2 (p = 0.03) and HSF1 content were decreased,reaching levels similar to those found in trained. Finally, miR-206 increased in trained EVs 24h after the exercise (p =0.045). These results suggest that the protein and myomiRcontent in the EVs’ cargo could reflect the tolerance toexercise at systemic level determined by the well-knownadaptation of the redox homeostasis components ormyomiRs in response to exercise in skeletal muscle and other tissues.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
