A staining procedure used for simultaneously determining three different fibre types in single sections bovine, porcine or ovine skeletal muscle was modified for use with ostrich skeletal muscle. The muscle fibres of gastrocnemius pars externa, tibialis cranialis caput tibiale, tibialis cranialis caput femorale and fibularis longus tendo caudalis were studied. The histochemical results revealed the presence of three types of fibre only in the gastrocnemius pars externa muscle: fast-twitch glycolytic fibres (FG), fast-twitch oxidative glycolytic fibres (FOG) and slow-twitch oxidative fibres (SO), while in the other muscles the FG fibres were absent. The percentage distribution of fibres types showed a higher incidence of SO fibres compared to FOG fibres in tibialis cranialis caput femorale and tibialis cranialis caput tibiale muscles, while it was opposite in the case of the fibularis longus tendo caudalis muscle. In the gastrocnemius pars externa muscle the FG fibres outnumber the other fibres, followed by the SO and FOG fibres. The results of the analysis of variance show significant interaction between muscle x fibre type for every morphometric parameter evaluated. Differences about value of fibres area exists between tibialis cranialis caput femorale and fibularis longus tendo caudalis muscles. Both fibre types in tibialis cranialis caput tibiale muscle have mean values of transversal section area smaller than tibialis cranialis caput femorale. The other morphometric parameters show a similar trend. The gastrocnemius pars externa muscle presents similar dimensions of muscle fibres for the FG and FOG types, and significantly smaller for the SO type.
File in questo prodotto:
Non ci sono file associati a questo prodotto.